Issue 9, 2013

Traceless protein delivery with an efficient recyclable nanocarrier

Abstract

Intracellular delivery is a prerequisite for the efficacy of many pharmaceutical proteins. Herein, vitamin B6 (pyridoxal-5′-phosphate, PLP) functionalized calcium phosphate (CP) is used as the bio-recyclable nanocarrier for delivery of proteins into cells. Proteins could be loaded on/released from PLP-CP via formation/hydrolysis of pH sensitive aldimine bridging lysine and surface-displayed PLP. The loaded proteins could be delivered into the cytosol of HeLa, HepG2 and L929 cells where the carrier could be metabolized into endogenous metabolites of Ca2+, HPO42−, and vitamin B6. PLP-CP mediated cell transduction is 10–40 folds more efficient than TAT which is a widely used cell penetrating peptide, demonstrating the utility of PLP-CP as the traceless platform for high-efficiency delivery of proteins into mammalian cells.

Graphical abstract: Traceless protein delivery with an efficient recyclable nanocarrier

Supplementary files

Article information

Article type
Paper
Submitted
14 May 2013
Accepted
06 Jun 2013
First published
03 Jul 2013

Biomater. Sci., 2013,1, 918-923

Traceless protein delivery with an efficient recyclable nanocarrier

X. Wu, Y. Song, J. Han, L. Yang and S. Han, Biomater. Sci., 2013, 1, 918 DOI: 10.1039/C3BM60128E

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements