Issue 19, 2013

Self-assembled multivalent RGD-peptide arrays – morphological control and integrin binding

Abstract

We report the synthesis of four different RGD peptide derivatives which spontaneously self-assemble into nanoscale architectures. Depending on the information programmed into the molecular-scale building blocks by organic synthesis, these compounds assemble into different nanoscale morphologies. This process can be fully understood using multiscale modelling which provides predictive insight into subtle differences, such as whether the compounds form spherical micelles, rod-like cylinders or tubular assemblies, and predicts experimentally observed critical aggregation concentrations (CACs). We then probe the multivalent binding of these assemblies to integrin proteins and demonstrate that the spherical micellar assemblies perform well in our solution-phase integrin binding assay as a consequence of self-assembled multivalency, with the CAC switching-on the binding. Conversely, the cylindrical assemblies do not work in this assay. As such, the nanoscale morphology controls the apparent ability to perform as a self-assembled multivalent ligand array.

Graphical abstract: Self-assembled multivalent RGD-peptide arrays – morphological control and integrin binding

Supplementary files

Article information

Article type
Paper
Submitted
08 Jan 2013
Accepted
22 Mar 2013
First published
05 Apr 2013

Org. Biomol. Chem., 2013,11, 3177-3186

Self-assembled multivalent RGD-peptide arrays – morphological control and integrin binding

D. J. Welsh, P. Posocco, S. Pricl and D. K. Smith, Org. Biomol. Chem., 2013, 11, 3177 DOI: 10.1039/C3OB00034F

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