Issue 37, 2013

Immobilization of polybia-MPI by allyl glycidyl ether based brush chemistry to generate a novel antimicrobial surface

Abstract

Significant efforts towards the covalent immobilization of antimicrobial peptides (AMPs) on biomaterial surfaces are under way to render implantable biodevices with antimicrobial functionalities. Towards this aim, we studied the potential and effectiveness of a novel AMP candidate, polybia-MPI (Pmpi), for immobilization on a silicon substrate using allyl glycidyl ether (AGE) based polymerization chemistry. A statistical Design of Experiment (DoE) platform was developed to quantitatively understand the effects of different immobilization parameters on the final tethered peptide surface concentration. Our platform demonstrates efficient tethering of Pmpi up to ∼10 μg cm−2 on silicon wafers. Concentration dependent antimicrobial activities of the ‘AGE-Pmpi’-tethered surfaces were observed, where a 70% reduction in bacterial colonies was achieved at a Pmpi surface concentration of 4.47 μg cm−2. The ‘AGE-Pmpi’-tethered surfaces retained their antibacterial property after 3 days of incubation in artificial urine, and a 4-fold reduction in biofilm formation was also evident, as determined by ellipsometry. FESEM and ATP leakage assay showed that the immobilized Pmpi compromised bacterial membrane integrity but showed negligible cytotoxicity against human red blood cells.

Graphical abstract: Immobilization of polybia-MPI by allyl glycidyl ether based brush chemistry to generate a novel antimicrobial surface

Supplementary files

Article information

Article type
Paper
Submitted
06 Jun 2013
Accepted
25 Jul 2013
First published
29 Jul 2013

J. Mater. Chem. B, 2013,1, 4746-4755

Immobilization of polybia-MPI by allyl glycidyl ether based brush chemistry to generate a novel antimicrobial surface

A. Basu, B. Mishra and S. S. Jan Leong, J. Mater. Chem. B, 2013, 1, 4746 DOI: 10.1039/C3TB20805B

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