Issue 25, 2016

Synthesis of the rare disaccharide nigerose by structure-based design of a phosphorylase mutant with altered regioselectivity

Abstract

In the absence of the natural acceptor inorganic phosphate wild-type sucrose phosphorylase from Bifidobacterium adolescentis (BaSP) produces maltose (4-O-α-D-glucopyranosyl-D-glucose) and kojibiose (2-O-α-D-glucopyranosyl-D-glucose) as sole transfer products. A Q345F exchange switches the enzyme's regioselectivity from 2 to 3 exclusively, yielding the rare sugar nigerose (3-O-α-D-glucopyranosyl-D-glucose, sakebiose).

Graphical abstract: Synthesis of the rare disaccharide nigerose by structure-based design of a phosphorylase mutant with altered regioselectivity

Supplementary files

Article information

Article type
Communication
Submitted
29 Jan 2016
Accepted
09 Feb 2016
First published
10 Feb 2016

Chem. Commun., 2016,52, 4625-4627

Synthesis of the rare disaccharide nigerose by structure-based design of a phosphorylase mutant with altered regioselectivity

M. Kraus, J. Görl, M. Timm and J. Seibel, Chem. Commun., 2016, 52, 4625 DOI: 10.1039/C6CC00934D

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements