Issue 51, 2018
From the journal:

Chemical Communications

Label-free and high-throughput bioluminescence detection of uracil-DNA glycosylase in cancer cells through tricyclic cascade signal amplification†

Yan Zhang,‡a   Qing-nan Li,‡a   Chen-chen Li‡a  and  Chun-yang Zhang ORCID logo *a  

* Corresponding authors

a College of Chemistry, Chemical Engineering and Materials Science, Collaborative Innovation Center of Functionalized Probes for Chemical Imaging in Universities of Shandong, Key Laboratory of Molecular and Nano Probes, Ministry of Education, Shandong Provincial Key Laboratory of Clean Production of Fine Chemicals, Shandong Normal University, Jinan 250014, China
E-mail: cyzhang@sdnu.edu.cn
Fax: +86 0531-82615258
Tel: +86 0531-86186033

Abstract

We develop a label-free and high-throughput bioluminescence method for the sensitive detection of uracil DNA glycosylase (UDG) through enzyme-mediated tricyclic cascade signal amplification. This method exhibits high sensitivity with a detection limit as low as 0.00031 U mL−1, and it can be further applied for the measurement of enzyme kinetic parameters and the screening of UDG inhibitors as well as cancer cell analysis.

Graphical abstract: Label-free and high-throughput bioluminescence detection of uracil-DNA glycosylase in cancer cells through tricyclic cascade signal amplification

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Article information

DOI
https://doi.org/10.1039/C8CC03769H
Article type
Communication
Submitted
10 May 2018
Accepted
16 May 2018
First published
16 May 2018

Chem. Commun., 2018,54, 6991-6994

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Label-free and high-throughput bioluminescence detection of uracil-DNA glycosylase in cancer cells through tricyclic cascade signal amplification

Y. Zhang, Q. Li, C. Li and C. Zhang, Chem. Commun., 2018, 54, 6991 DOI: 10.1039/C8CC03769H

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