Abstract

A novel biosensor for the amperometric detection of glutathione is proposed in which glutathione reductase is coupled to a pyrrolofullerene bis-adduct playing the role of redox mediator between electrode and enzyme. The adoption of a simple procedure for realizing the immobilization of the redox mediator, together with the enzyme, within a polypyrrole film cast onto a glassy carbon electrode, was made possible by the amphiphilic properties of the fullerene derivative. The biosensor showed a fast and reproducible response to glutathione and, because of its lower sensitivity, a wider linear range with respect to a similar device based on a diffusional redox mediator.