Issue 4, 2003
From the journal:

Lab on a Chip

GlycoChip: multiarray for the study of carbohydrate-binding proteins

O. E. Galanina,a   M. Mecklenburg,b   N. E. Nifantiev,c   G. V. Pazyninaa  and  N. V. Bovin*a  

* Corresponding authors

a Shemyakin & Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Miklukho-Maklaya 16/10, 117997 Moscow, Russia
E-mail: bovin@carb.siobc.ras.ru
Fax: 7 (095) 330-55-92
Tel: 7 (095) 330-71-38

b Interactiva GmbH, Ulm, Germany

c Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia

Abstract

Biotinylated glycoconjugates which were designed as oligosaccharides attached to 30 kDa polyacrylamide were coated on a microarray platform XNAonGOLD™, which was developed earlier for an oligonucleotide assay. The specificity of antibodies to carbohydrate antigens was analyzed using the glyco-microarray. Comparison of the obtained results with those of common 96-well plate ELISA completely coincided with the found antibody specificities. However, parameters such as the analytical sensitivity of the method and the amount of biotinylated material coated on the microarray platform proved to be worse than expected.

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Article information

DOI
https://doi.org/10.1039/B305963D
Article type
Paper
Submitted
27 May 2003
Accepted
01 Aug 2003
First published
18 Aug 2003

Lab Chip, 2003,3, 260-265

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GlycoChip: multiarray for the study of carbohydrate-binding proteins

O. E. Galanina, M. Mecklenburg, N. E. Nifantiev, G. V. Pazynina and N. V. Bovin, Lab Chip, 2003, 3, 260 DOI: 10.1039/B305963D

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