Issue 5, 2005

Dried-droplet laser ablation ICP-MS of HPLC fractions for the determination of selenomethionine in yeast

Abstract

Offline coupling of high performance liquid chromatography (HPLC) to dried droplet laser ablation (LA) ICP-MS detection is described. The method is based on the offline spotting of HPLC fractions onto a substrate followed by LA introduction of the dried sample residues to an ICP-MS for elemental analysis. Quantitation of selenomethionine (SeMet) in yeast using species specific isotope dilution (ID) was achieved following digestion of samples in 4 M methanesulfonic acid and HPLC separation of species. Chromatographic fractions having retention times of 180–210 seconds were collected for each sample. Dried micro-droplets from each fraction were ablated from a polystyrene substrate and quantitated for SeMet. Concentrations of 3301 ± 18 and 3309 ± 24 μg g−1 (one standard deviation, n = 4) with RSDs of 0.55% and 0.73% were obtained based on measured 78Se/74Se and 82Se/74Se ratios, in good agreement with the values of 3309 ± 19 and 3305 ± 26 μg g−1 (one standard deviation, n = 4, RSDs of 0.58% and 0.79%), respectively, obtained by direct HPLC-ICP-MS analysis. The proposed method provides a satisfactory alternative technique for the quantitation of SeMet in yeast.

Graphical abstract: Dried-droplet laser ablation ICP-MS of HPLC fractions for the determination of selenomethionine in yeast

Article information

Article type
Paper
Submitted
19 Jan 2005
Accepted
14 Mar 2005
First published
01 Apr 2005

J. Anal. At. Spectrom., 2005,20, 431-435

Dried-droplet laser ablation ICP-MS of HPLC fractions for the determination of selenomethionine in yeast

L. Yang, R. E. Sturgeon and Z. Mester, J. Anal. At. Spectrom., 2005, 20, 431 DOI: 10.1039/B500926J

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