Targeting of mixed sequence double-stranded DNA using pyrene-functionalized 2′-amino-α-L-LNA

Patrick J. Hrdlicka; T. Santhosh Kumar; Jesper Wengel

doi:10.1039/B506986F

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Targeting of mixed sequence double-stranded DNA using pyrene-functionalized 2′-amino-α-l-LNA†

Patrick J. Hrdlicka,^a T. Santhosh Kumar^a and Jesper Wengel*^a

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* Corresponding authors

^a Nucleic Acid Center, Department of Chemistry, University of Southern Denmark, Denmark
E-mail: jwe@chem.sdu.dk
Fax: (+45) 6615 8780
Tel: (+45) 6550 2510

Abstract

Incorporation of a single pyrene-functionalized 2′-amino-α-L-LNA monomer X into short DNA strands induces extraordinarily high binding affinity towards complementary DNA (up to 16 °C increase per modification), whereas labile duplexes, suitable as probes for targeting of double stranded DNA, are formed upon positioning of two monomers X in an interstrand +1 zipper motif.

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Supplementary files

Discussion regarding synthesis of nucleosides 1 - 3 and ON1 - ON7, details of thermal denaturation, fluorescence, dsDNA recognition and molecular modelling experiments, absorption maxima of oligonucleotides (Table S1), lowest energy structure of ON4: ON7 from molecular modelling (Fig. S1) and additional steady-state fluorescence emission spectra of molecular recognition experiments (Fig. S2) PDF (507K)

Article information

DOI: https://doi.org/10.1039/B506986F
Article type: Communication
Submitted: 17 May 2005
Accepted: 15 Jun 2005
First published: 21 Jul 2005

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Chem. Commun., 2005, 4279-4281

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Targeting of mixed sequence double-stranded DNA using pyrene-functionalized 2′-amino-α-L-LNA

P. J. Hrdlicka, T. S. Kumar and J. Wengel, Chem. Commun., 2005, 4279 DOI: 10.1039/B506986F

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Chemical Communications

Targeting of mixed sequence double-stranded DNA using pyrene-functionalized 2′-amino-α-l-LNA†

Abstract

Supplementary files

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Targeting of mixed sequence double-stranded DNA using pyrene-functionalized 2′-amino-α-L-LNA

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