Issue 2, 2006

Monitoring cyclodextrin–polyviologen pseudopolyrotaxanes with the Bradford assay

Abstract

Self-assembled multivalent pseudopolyrotaxanes, composed of lactoside-bearing cyclodextrin (CD) rings threaded on linear polyviologen polymers, have been introduced recently as flexible and dynamic neoglycoconjugates. In the course of this research, it was found that polyviologens are responsive to the Bradford assay, which is traditionally highly selective for proteins. The response of the pseudopolyrotaxanes to the Bradford assay was dependant on, and thus indicative of, the degree of threading of the CD rings onto the polyelectrolyte. The assay was then used to report on the threading and dethreading of native and lactoside-bearing α-CD rings onto and off of polyviologen chains, a phenomenon which demonstrates the utility of biochemical assays to address problems unique to supramolecular chemistry.

Graphical abstract: Monitoring cyclodextrin–polyviologen pseudopolyrotaxanes with the Bradford assay

Article information

Article type
Paper
Submitted
08 Jul 2005
Accepted
22 Nov 2005
First published
09 Dec 2005

Org. Biomol. Chem., 2006,4, 250-256

Monitoring cyclodextrin–polyviologen pseudopolyrotaxanes with the Bradford assay

J. M. Belitsky, A. Nelson and J. F. Stoddart, Org. Biomol. Chem., 2006, 4, 250 DOI: 10.1039/B509576J

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