Issue 3, 2006
From the journal:

Green Chemistry

Structure and activity of Candida antarctica lipase B in ionic liquids†

Fred van Rantwijk,a   Francesco Secundob  and  Roger A. Sheldon*a  

* Corresponding authors

a Laboratory of Biocatalysis and Organic Chemistry, Delft University of Technology, Julianalaan 136, The Netherlands
E-mail: r.a.sheldon@tnw.tudelft.nl

b Istituto di Chimica del Riconoscimento Moleculare, CNR, Via Mario Bianco 9, Milano, Italy
E-mail: francesco.secundo@icrm.cnr.it

Abstract

The effects of ionic liquid media on the activity of Candida antarctica lipase B in a simple transesterification reaction were studied. In ionic liquids containing dicyanimide, alkylsulfate, nitrate and lactate anions, which dissolved CaLB, the reaction was at least ten times slower than in [BMIm][BF4]. Only [Et3MeN][MeSO4] was an exception, as dissolved CaLB maintained its activity in this solvent. A cross-linked enzyme aggregate of CaLB was twice as active in [BMIm][dca] as in tert-butyl alcohol, whereas the free enzyme was irreversibly deactivated in this latter ionic liquid.

Graphical abstract: Structure and activity of Candida antarctica lipase B in ionic liquids

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Article information

DOI
https://doi.org/10.1039/B513062J
Article type
Paper
Submitted
14 Sep 2005
Accepted
08 Nov 2005
First published
01 Dec 2005

Green Chem., 2006,8, 282-286

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Structure and activity of Candida antarctica lipase B in ionic liquids

F. van Rantwijk, F. Secundo and R. A. Sheldon, Green Chem., 2006, 8, 282 DOI: 10.1039/B513062J

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