Issue 8, 2006

A novel miniature cell retainer for correlative high-content analysis of individual untethered non-adherent cells

Abstract

The importance of research involving non-adherent cell lines, primary cells and blood cells is generally undisputed. However, the task of investigating the complexity and heterogeneity of these cells calls for their long-run monitoring at a single-cell resolution. Such a capability is currently unavailable without having to use disruptive cell tethering. The present Cell Retainer (CR) concept enables high-content correlative multi-parametric measurements, from the functional to molecular level, of the same living individual non-adherent cells within a population. Thereby, despite extensive long-term bio-manipulations, the cells preserve their identity without tethering. Several exemplary experiments, using a microscope-slide-based version of the CR, are presented, which could not be performed by other state of the art methods.

Graphical abstract: A novel miniature cell retainer for correlative high-content analysis of individual untethered non-adherent cells

Supplementary files

Article information

Article type
Paper
Submitted
16 Mar 2006
Accepted
17 May 2006
First published
02 Jun 2006

Lab Chip, 2006,6, 995-1000

A novel miniature cell retainer for correlative high-content analysis of individual untethered non-adherent cells

M. Deutsch, A. Deutsch, O. Shirihai, I. Hurevich, E. Afrimzon, Y. Shafran and N. Zurgil, Lab Chip, 2006, 6, 995 DOI: 10.1039/B603961H

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