Enhancement of RNA cleavage activity of 10–23 DNAzyme by covalently introduced intercalator

Hiroyuki Asanuma; Hiroyuki Hayashi; Jing Zhao; Xingguo Liang; Akira Yamazawa; Takeshi Kuramochi; Daijiro Matsunaga; Yuichiro Aiba; Hiromu Kashida; Makoto Komiyama

doi:10.1039/B611078A

Enhancement of RNA cleavage activity of 10–23 DNAzyme by covalently introduced intercalator†

Hiroyuki Asanuma,*^ab Hiroyuki Hayashi,^a Jing Zhao,^b Xingguo Liang,^a Akira Yamazawa,^c Takeshi Kuramochi,^c Daijiro Matsunaga,^c Yuichiro Aiba,^c Hiromu Kashida^c and Makoto Komiyama^c

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* Corresponding authors

^a Department of Molecular Design and Engineering, Graduate School of Engineering, Nagoya University, Chikusa-ku, Japan
E-mail: asanuma@mol.nagoya-u.ac.jp

^b PRESTO, Japan Science and Technology Agency (JST), Kawaguchi 332-0012, Japan

^c RCAST, The University of Tokyo, 4-6-1 Komaba, Meguro-ku, Japan

Abstract

By introducing an intercalator through D-threoninol to the 10–23 DNAzyme at the junction between its catalytic loop and the binding arm, the RNA cleavage activity was greatly improved.

Chemical Communications

Enhancement of RNA cleavage activity of 10–23 DNAzyme by covalently introduced intercalator†

Abstract

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Enhancement of RNA cleavage activity of 10–23 DNAzyme by covalently introduced intercalator

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