Issue 7, 2007
From the journal:

Chemical Communications

Construction of di-scFv through a trivalent alkyne–azide 1,3-dipolar cycloaddition†

Arutselvan Natarajan,‡a   Wenjun Du,‡§b   Cheng-Yi Xiong,a   Gerald L. DeNardo,a   Sally J. DeNardo*a  and  Jacquelyn Gervay-Hague*b  

* Corresponding authors

a Radiodiagnosis and Therapy, Molecular Cancer Institute, University of California, Davis Medical Center, 1508 Alhambra Boulevard, Sacramento, USA

b Department of Chemistry, University of California, Davis, One Shields Avenue, Davis, USA
E-mail: gervay@chem.ucdavis.edu
Fax: +1 530 752- 8995
Tel: +1 530 754 9577

Abstract

Heterofunctional azide and alkyne PEG-linkers have been synthesized and site specifically conjugated to scFv via a reactive thiol functionality; two scFv were coupled by copper catalyzed 1,3-dipolar cycloaddition to make divalent scFv (di-scFv) with an inter-scFv distance defined to provide divalent binding; antigen binding was maintained for the di-scFv construct and increased several times compared to that of the parent scFv; the cycloaddition reaction reported herein represents an important ligation strategy to covalently link macromolecular proteins and retain sensitive structural conformations.

Graphical abstract: Construction of di-scFv through a trivalent alkyne–azide 1,3-dipolar cycloaddition

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Article information

DOI
https://doi.org/10.1039/B611636A
Article type
Communication
Submitted
11 Aug 2006
Accepted
30 Oct 2006
First published
28 Nov 2006

Chem. Commun., 2007, 695-697

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Construction of di-scFv through a trivalent alkyne–azide 1,3-dipolar cycloaddition

A. Natarajan, W. Du, C. Xiong, G. L. DeNardo, S. J. DeNardo and J. Gervay-Hague, Chem. Commun., 2007, 695 DOI: 10.1039/B611636A

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