Issue 3, 2007

A plate reader-compatible microchannel array for cell biology assays

Abstract

The use of microfluidic devices for studying cell biology holds considerable promise when compared to canonical culture systems. However, the lack of compatibility with existing infrastructure hinders the application of microfluidic devices in the life sciences. Here, we present a microchannel cell culture platform having both operational (compatible with plate readers and pipetting) and performance (lower detection limits, controlled microenvironment) advantages. We demonstrated rapid growth assays and immunocytochemistry on mammary epithelial cells (both a cell line and primary cells) in the microchannel arrays. The utilization of ubiquitous pipetting methods and plate reader endpoints lowers the barriers to use. The simplicity and flexibility of the platform, combined with the throughput of automated detection systems, will facilitate the adoption of microfluidic culture systems in biological laboratories.

Graphical abstract: A plate reader-compatible microchannel array for cell biology assays

Supplementary files

Article information

Article type
Technical Note
Submitted
01 Sep 2006
Accepted
08 Dec 2006
First published
02 Jan 2007

Lab Chip, 2007,7, 388-391

A plate reader-compatible microchannel array for cell biology assays

H. Yu, C. M. Alexander and D. J. Beebe, Lab Chip, 2007, 7, 388 DOI: 10.1039/B612358A

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements