Issue 26, 2007

Consecutive GC base pairs determine the energy barrier of DNA duplex formation under molecularly crowded conditions

Abstract

The kinetics of DNA duplex formation was affected by the addition of PEGs with different masses (MW = 200–8000) to an aqueous solution; for each condition, two duplexes (5′-TAGGTTATAA-3′/5′-TTATAACCTA-3′ and 5′-CAGGTCACAG-3′/5′-CTGTGACCTG-3′) with different stabilities were formed after overcoming the same association activation energy barrier, suggesting that the formation of consecutive GC base pairs in the helices rather than the helix terminus is the initiation nucleus for DNA duplex formation not only in the absence, but also in the presence of PEGs.

Graphical abstract: Consecutive GC base pairs determine the energy barrier of DNA duplex formation under molecularly crowded conditions

Supplementary files

Article information

Article type
Communication
Submitted
26 Feb 2007
Accepted
10 Apr 2007
First published
23 Apr 2007

Chem. Commun., 2007, 2750-2752

Consecutive GC base pairs determine the energy barrier of DNA duplex formation under molecularly crowded conditions

X. Gu, S. Nakano and N. Sugimoto, Chem. Commun., 2007, 2750 DOI: 10.1039/B702865B

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