Issue 10, 2007

Population patch clamp electrophysiology: a breakthrough technology for ion channel screening

Abstract

Population patch clamp (PPC) is a novel high throughput planar array electrophysiology technique that allows ionic currents to be recorded from populations of cells under voltage clamp. For the drug discovery pharmacologist, PPC promises greater speed and precision than existing methods for screening compounds at voltage-gated ion channel targets. Moreover, certain constitutively active or slow-ligand gated channels that have hitherto proved challenging to screen with planar array electrophysiology (e.g. SK/IK channels) are now more accessible. In this article we review early findings using PPC and provide a perspective on its likely impact on ion channel drug discovery. To support this, we include some new data on ion channelassay duplexing and on modulator assays, approaches that have thus far not been described.

Graphical abstract: Population patch clamp electrophysiology: a breakthrough technology for ion channel screening

Article information

Article type
Paper
Submitted
23 Apr 2007
Accepted
30 May 2007
First published
15 Jun 2007

Mol. BioSyst., 2007,3, 714-722

Population patch clamp electrophysiology: a breakthrough technology for ion channel screening

T. J. Dale, C. Townsend, E. C. Hollands and D. J. Trezise, Mol. BioSyst., 2007, 3, 714 DOI: 10.1039/B706152H

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