Issue 7, 2008

Chemical synthesis and semisynthesis of membrane proteins

Abstract

Total chemical synthesis and semisynthesis of proteins have become widely used tools to alter and control the chemical structure of soluble proteins, Thus, offering unique possibilities to understand protein function in vitro and in vivo. However, these approaches rely on our ability to produce and chemoselectively link peptide segments with each other or with recombinantly produced protein segments. Access to integral membrane and membrane-associated proteinsvia these approaches has been hampered by the fact that integral membrane peptides or lipid-modified peptides are difficult to obtain mostly due to incomplete amino acid coupling reactions and their poor handling properties. This article will highlight the advances in the total chemical synthesis and semisynthesis of small viral as well as bacterial ion channels. Recent synthesis approaches for membrane-associated proteins will be discussed as well.

Graphical abstract: Chemical synthesis and semisynthesis of membrane proteins

Article information

Article type
Review Article
Submitted
25 Feb 2008
Accepted
02 Apr 2008
First published
27 May 2008

Mol. BioSyst., 2008,4, 733-740

Chemical synthesis and semisynthesis of membrane proteins

D. Olschewski and C. F. W. Becker, Mol. BioSyst., 2008, 4, 733 DOI: 10.1039/B803248C

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