Issue 8, 2008

Electrochemical impedance spectroscopy and surface plasmon resonance studies of DNA hybridization on gold/SiOx interfaces

Abstract

The use of Au/SiOx interfaces for the investigation of DNA hybridization using electrochemical impedance spectroscopy (EIS) and surface plasmon resonance (SPR) simultaneously is demonstrated. Standard glass chemistry was used to link single-stranded DNA (ss-DNA) on aldehyde-terminated Au/SiOx interfaces. The layer thickness and amount of grafted oligonucleotides (ODNs) were calculated from SPR on the basis of a multilayer system of glass/Ti/Au/SiOx/grafted molecule. Capacitance and resistance values of the modified interface before and after hybridization were calculated from EIS data using an equivalent circuit and allowed the affinity rate constant, KA = 4.07 × 105 M−1, to be determined. The EIS results were comparable to those obtained by SPR hybridization kinetics recorded in parallel.

Graphical abstract: Electrochemical impedance spectroscopy and surface plasmon resonance studies of DNA hybridization on gold/SiOx interfaces

Article information

Article type
Paper
Submitted
20 Mar 2008
Accepted
09 May 2008
First published
01 Jul 2008

Analyst, 2008,133, 1097-1103

Electrochemical impedance spectroscopy and surface plasmon resonance studies of DNA hybridization on gold/SiOx interfaces

M. Manesse, V. Stambouli, R. Boukherroub and S. Szunerits, Analyst, 2008, 133, 1097 DOI: 10.1039/B804825H

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