Issue 45, 2008
From the journal:

Chemical Communications

A high sensitive and specific QDs FRET bioprobe for MNase†

Shan Huang,‡a   Qi Xiao,‡b   Zhi Ke He,*a   Yi Liu,b   Philip Tinnefeld,c   Xiong Rui Sud  and  Xiao Niu Pengd  

* Corresponding authors

a Research Center of Analytical Science, College of Chemistry and Molecular Sciences, Wuhan University, Wuhan, PR China
E-mail: zhkhe@whu.edu.cn
Fax: +86-27-6875-4067
Tel: +86-27-8716-2672

b State Key Laboratory of Virology, College of Chemistry and Molecular Science, Wuhan University, Wuhan, PR China

c Applied Physics, Department of Applied Physics-Biophysics, and Center for NanoScience, Ludwig-Maximilians Universität München, Amalienstrasse 54, München, Germany

d Department of Physics and Key Laboratory of Acoustic and Photonic Materials and Devices of Ministry of Education, Wuhan University, Wuhan, PR China

Abstract

We used CdSe/ZnS quantum dots–ssDNA–fluorescent dye conjugates as bioprobes to detect micrococcal nuclease with high specificity and sensitivity, and further utilized the bioprobe to monitor the micrococcal nuclease activity in the culture medium of Staphylococcus aureus by fluorescence microscopy.

Graphical abstract: A high sensitive and specific QDs FRET bioprobe for MNase

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Article information

DOI
https://doi.org/10.1039/B815061C
Article type
Communication
Submitted
29 Aug 2008
Accepted
23 Sep 2008
First published
14 Oct 2008

Chem. Commun., 2008, 5990-5992

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A high sensitive and specific QDs FRET bioprobe for MNase

S. Huang, Q. Xiao, Z. K. He, Y. Liu, P. Tinnefeld, X. R. Su and X. N. Peng, Chem. Commun., 2008, 5990 DOI: 10.1039/B815061C

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