Issue 8, 2009

Controlling the selection stringency of phage display using a microfluidic device

Abstract

We report the utilization of microfluidic technology to phage selection and demonstrate that accurate control of washing stringency in our microfluidic magnetic separator (MMS) directly impacts the diversity of isolated peptide sequences. Reproducible generation of magnetic and fluidic forces allows controlled washing conditions that enable rapid convergence of selected peptide sequences. These findings may provide a foundation for the development of automated microsystems for rapid in vitro directed evolution of affinity reagents.

Graphical abstract: Controlling the selection stringency of phage display using a microfluidic device

Additions and corrections

Article information

Article type
Communication
Submitted
24 Nov 2008
Accepted
18 Feb 2009
First published
03 Mar 2009

Lab Chip, 2009,9, 1033-1036

Controlling the selection stringency of phage display using a microfluidic device

Y. Liu, J. D. Adams, K. Turner, F. V. Cochran, S. S. Gambhir and H. T. Soh, Lab Chip, 2009, 9, 1033 DOI: 10.1039/B820985E

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