Issue 35, 2009

Novel nanocomposites consisting of in vivo-biotinylated bacterial magnetic particles and quantum dots for magnetic separation and fluorescent labeling of cancer cells

Abstract

Novel nanocomposites consisting of nano-sized bacterial magnetic particles (BacMPs) and semiconductor quantum dots (QD) were developed for use in targeting and identifying cancer cells. BacMPs are synthesized by magnetotactic bacterium, Magnetospirillum magneticumAMB-1 and can display functional proteins using gene fusion techniques. In this study, two functional proteins, biotin carboxyl carrier protein (BCCP), derived from AMB-1 and protein G, derived from Streptococcus were displayed on BacMPs. BCCP was biotinylated in AMB-1 cells by endogenous biotin ligase. After purification of in vivo-biotinylated BacMPs, streptavidin and antibodies were immobilized on protein G-BCCP-displaying BacMPs (protein G-BCCP-BacMPs) viabiotin and protein G, respectively. Furthermore, multi-color labeling of the protein G-BCCP-BacMPs was achieved with streptavidin-labeled QD. Using streptavidin-QD/protein G-BCCP-BacMP nanocomposites, we successfully demonstrated magnetic manipulation and fluorescent labeling of lung cancer cells.

Graphical abstract: Novel nanocomposites consisting of in vivo-biotinylated bacterial magnetic particles and quantum dots for magnetic separation and fluorescent labeling of cancer cells

Article information

Article type
Paper
Submitted
13 Jan 2009
Accepted
24 Mar 2009
First published
20 Apr 2009

J. Mater. Chem., 2009,19, 6361-6366

Novel nanocomposites consisting of in vivo-biotinylated bacterial magnetic particles and quantum dots for magnetic separation and fluorescent labeling of cancer cells

Y. Maeda, T. Yoshino and T. Matsunaga, J. Mater. Chem., 2009, 19, 6361 DOI: 10.1039/B900693A

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