Issue 32, 2009

Monoclonal antibody-functionalized mesoporous silicananoparticles (MSN) for selective targeting breast cancer cells

Abstract

In this work, we conjugate anti-HER2/neu mAb (monoclonal antibody) to green fluorescent dye loaded mesoporous silica nanoparticles (Her-Dye@MSN) through a polyethylene glycol (MW = 5000) spacer. We examine their targeting properties toward HER2/neu over-expressing breast cancer cells and their internalization into the cells. Her-Dye@MSN nanoparticles exhibit a high targeting efficiency and the specific targeting capability is strongly affected by the mAb density on the Her-Dye@MSN. The time-course of competitive experiments with free antibody indicates that Her-Dye@MSN serves as a multivalent ligand. Moreover, Her-Dye@MSNs are internalized into cells through a receptor-mediated endocytosis and may escape from endosome to cytosol. The results reported here further support the potential of MSNs as a multifunctional smart nanocarriers for cell imaging and drug delivery.

Graphical abstract: Monoclonal antibody-functionalized mesoporous silica nanoparticles (MSN) for selective targeting breast cancer cells

Supplementary files

Article information

Article type
Paper
Submitted
13 Mar 2009
Accepted
14 May 2009
First published
24 Jun 2009

J. Mater. Chem., 2009,19, 5737-5743

Monoclonal antibody-functionalized mesoporous silica nanoparticles (MSN) for selective targeting breast cancer cells

C. Tsai, C. Chen, Y. Hung, F. Chang and C. Mou, J. Mater. Chem., 2009, 19, 5737 DOI: 10.1039/B905158A

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Spotlight

Advertisements