An integrated microfluidic device for large-scale in situ click chemistry screening

Yanju Wang; Wei-Yu Lin; Kan Liu; Rachel J. Lin; Matthias Selke; Hartmuth C. Kolb; Nangang Zhang; Xing-Zhong Zhao; Michael E. Phelps; Clifton K. F. Shen; Kym F. Faull; Hsian-Rong Tseng

doi:10.1039/B907430A

An integrated microfluidic device for large-scale in situ click chemistry screening†

Yanju Wang,‡^ac Wei-Yu Lin,‡^a Kan Liu,^a Rachel J. Lin,^a Matthias Selke,^c Hartmuth C. Kolb,^d Nangang Zhang,^ae Xing-Zhong Zhao,^e Michael E. Phelps,^a Clifton K. F. Shen,^a Kym F. Faull*^b and Hsian-Rong Tseng*^a

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* Corresponding authors

^a Crump Institute for Molecular Imaging, Department of Molecular and Medical Pharmacology, David Geffen School of Medicine at UCLA, 570 Westwood Plaza, building 114, Los Angeles, CA, USA
E-mail: hrtseng@mednet.ucla.edu

^b Pasarow Mass Spectrometry Laboratory, Department of Psychiatry & Biobehavioral Sciences and the Semel Institute for Neuroscience and Human Behavior, David Geffen School of Medicine at UCLA, Los Angeles, CA, USA
E-mail: faull@chem.ucla.edu

^c Department of Chemistry and Biochemistry, California State University, Los Angeles, 5151 State University Drive, Los Angeles, CA

^d Siemens Medical Solutions USA, Inc., Bristol Parkway, Culver City, CA, USA

^e Department of Physics, School of Physics, Center of Nanoscience and Nanotechnology, Wuhan University, Wuhan, China

Abstract

An integrated microfluidic device has been developed to perform 1024 in situ click chemistry reactions in parallel using the bovine carbonic anhydrous II (bCAII) click chemistry system as a proof-of-concept study and a rapid hit identification approach using SPE purification and electrospray-ionization mass spectrometry, multiple reaction monitoring (MRM) analysis, all of which improves the sensitivity and throughput of the downstream analysis.

Lab on a Chip

An integrated microfluidic device for large-scale in situ click chemistry screening†

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An integrated microfluidic device for large-scale in situ click chemistry screening

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