Issue 39, 2009

Site-specific covalent labeling of His-tag fused proteins with a reactive Ni(ii)–NTA probe

Abstract

A new method for covalent labeling of a His-tag fused protein with a small reactive probe was developed; this method is based on the complementary interaction between the His-tag and Ni(II)–NTA, which facilitates a nucleophilic reaction between a histidine residue of the tag and the electrophilictosyl group of the Ni(II)–NTA probe by the proximity effect.

Graphical abstract: Site-specific covalent labeling of His-tag fused proteins with a reactive Ni(ii)–NTA probe

Supplementary files

Article information

Article type
Communication
Submitted
18 Jun 2009
Accepted
22 Jul 2009
First published
13 Aug 2009

Chem. Commun., 2009, 5880-5882

Site-specific covalent labeling of His-tag fused proteins with a reactive Ni(II)–NTA probe

S. Uchinomiya, H. Nonaka, S. Fujishima, S. Tsukiji, A. Ojida and I. Hamachi, Chem. Commun., 2009, 5880 DOI: 10.1039/B912025D

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