Issue 1, 2010

Potassium-sensitive G-quadruplexDNA for sensitive visible potassium detection

Abstract

A novel visual method for K+ detection is developed utilizing a K+-sensitive G-quadruplex DNA named PS5.M as the sensing element. In the absence of K+, PS5.M is in a random coil state and does not bind hemin. Upon addition of K+, PS5.M folds into the quadruplex structure stabilized by K+. Such quadruplex structure is able to bind hemin to form the hemin-G-quadruplex DNAzyme that catalyzes the H2O2-mediated oxidation of colorless 3,3′,5,5′-tetramethylbenzidine to the blue product. Under the optimal conditions, the color change can be clearly observed with the naked eye in the concentration range from 2 to 1000 μM. Through this facile approach, K+ can be detected down to 2 μM, with high selectivity against other cations. In view of its simplicity, sensitivity and specificity, our developed visual method for K+ detection would potentially be utilized for bioassays and K+-sensitive test paper.

Graphical abstract: Potassium-sensitive G-quadruplex DNA for sensitive visible potassium detection

Supplementary files

Article information

Article type
Paper
Submitted
01 Jul 2009
Accepted
22 Oct 2009
First published
10 Nov 2009

Analyst, 2010,135, 71-75

Potassium-sensitive G-quadruplex DNA for sensitive visible potassium detection

X. Yang, T. Li, B. Li and E. Wang, Analyst, 2010, 135, 71 DOI: 10.1039/B913036E

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