Element-to-tissue correlation in biological samples determined by three-dimensional X-ray imaging methods

Björn De Samber; Geert Silversmit; Karel De Schamphelaere; Roel Evens; Tom Schoonjans; Bart Vekemans; Colin Janssen; Bert Masschaele; Luc Van Hoorebeke; Imre Szalóki; Frank Vanhaecke; Karen Rickers; Gerald Falkenberg; Laszlo Vincze

doi:10.1039/B918624G

Element-to-tissue correlation in biological samples determined by three-dimensional X-ray imaging methods

Björn De Samber,^a Geert Silversmit,^a Karel De Schamphelaere,^b Roel Evens,^b Tom Schoonjans,^a Bart Vekemans,^a Colin Janssen,^b Bert Masschaele,^c Luc Van Hoorebeke,^c Imre Szalóki,^d Frank Vanhaecke,^a Karen Rickers,^e Gerald Falkenberg^e and Laszlo Vincze*^a

Author affiliations

* Corresponding authors

^a Department of Analytical Chemistry, Ghent University, Krijgslaan 281, Ghent, Belgium
E-mail: Laszlo.Vincze@UGent.be
Fax: +3292646699
Tel: +3292644822

^b Laboratory of Environmental Toxicology and Aquatic Ecology, Ghent University, Jozef Plateaustraat 22, Ghent, Belgium

^c Centre for X-Ray Tomography, Department of Subatomic and Radiation Physics, Ghent University, Proeftuinstraat 86, Ghent, Belgium

^d Budapest University of Technology and Economics, Institute of Nuclear Techniques, Műegyetem rkp. 9, Budapest, Hungary

^e Hamburger Synchrotronstrahlungslabor at DESY, Notkestr. 85, Hamburg, Germany

Abstract

Synchrotron radiation based confocal micro-XRF was employed to unravel the tissue-specific three-dimensional (3D) distribution of metals down to trace concentration levels in a non-destructive manner within the crustacean Daphnia magna, an ecotoxicological model organism. Next to the analytical characterization of the employed confocal micro-XRF set-up, specific areas of metal accumulation in different cross-sections of interest within the organism were investigated. The use of a fast dynamic (continuous) scanning approach delivered 3D information on major, minor and trace element distributions in specific sub-regions within Daphnia magna. Coupling the obtained elemental information with microscopic morphological data obtained by laboratory absorption microtomography, full 3D element-to-tissue correlation could be derived, allowing a more detailed interpretation of the obtained results with respect to metal accumulation within this model organism.

Journal of Analytical Atomic Spectrometry

Element-to-tissue correlation in biological samples determined by three-dimensional X-ray imaging methods

Abstract

Article information

Download Citation

Permissions

Element-to-tissue correlation in biological samples determined by three-dimensional X-ray imaging methods

Social activity

Search articles by author

Spotlight

Advertisements