Issue 10, 2010

Quantitative mapping of aqueous microfluidic temperature with sub-degree resolution using fluorescence lifetime imaging microscopy

Abstract

The use of a water-soluble, thermo-responsive polymer as a highly sensitive fluorescence-lifetime probe of microfluidic temperature is demonstrated. The fluorescence lifetime of poly(N-isopropylacrylamide) labelled with a benzofurazan fluorophore is shown to have a steep dependence on temperature around the polymer phase transition and the photophysical origin of this response is established. The use of this unusual fluorescent probe in conjunction with fluorescence lifetime imaging microscopy (FLIM) enables the spatial variation of temperature in a microfluidic device to be mapped, on the micron scale, with a resolution of less than 0.1 °C. This represents an increase in temperature resolution of an order of magnitude over that achieved previously by FLIM of temperature-sensitive dyes.

Graphical abstract: Quantitative mapping of aqueous microfluidic temperature with sub-degree resolution using fluorescence lifetime imaging microscopy

Supplementary files

Article information

Article type
Paper
Submitted
17 Nov 2009
Accepted
03 Feb 2010
First published
25 Feb 2010

Lab Chip, 2010,10, 1267-1273

Quantitative mapping of aqueous microfluidic temperature with sub-degree resolution using fluorescence lifetime imaging microscopy

E. M. Graham, K. Iwai, S. Uchiyama, A. Prasanna de Silva, S. W. Magennis and A. C. Jones, Lab Chip, 2010, 10, 1267 DOI: 10.1039/B924151E

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