Issue 11, 2010

Differentiating quadruplexes: binding preferences of a luminescent dinuclear ruthenium(ii) complex with four-stranded DNA structures

Abstract

The interaction of luminescent dinuclear ruthenium(II) complexes containing the bridging ligand tetrapyrido[3,2-a:2′,3′-c:3′′,2′′-h:2′′,3′′-j]phenazine with several intramolecular and intermolecular quadruplex DNA structures has been explored. It was found that these interactions produced distinctly different luminescence signatures. Binding curves constructed from these optical changes reveal that binding affinities for the quadruplex structures vary by over two orders of magnitude. The differences in quadruplex binding affinity and optical signature are rationalized through a consideration of the structural features of the quadruplexes. In particular we conclude large blue shifted emission enhancements are only observed on binding to quadruplexes containing lateral loops that are at least three base pairs long.

Graphical abstract: Differentiating quadruplexes: binding preferences of a luminescent dinuclear ruthenium(ii) complex with four-stranded DNA structures

Article information

Article type
Paper
Submitted
19 Nov 2009
Accepted
12 Mar 2010
First published
29 Mar 2010

Org. Biomol. Chem., 2010,8, 2617-2621

Differentiating quadruplexes: binding preferences of a luminescent dinuclear ruthenium(II) complex with four-stranded DNA structures

T. Wilson, M. P. Williamson and J. A. Thomas, Org. Biomol. Chem., 2010, 8, 2617 DOI: 10.1039/B924263E

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