Issue 7, 2010

Three dimensional collagen gels as a cell culture matrix for the study of live cells by Raman spectroscopy

Abstract

Three dimensional collagen gels are evaluated as matrices for the study of live cells by Raman spectroscopy. The study is conducted on a human lung adenocarcinoma (A549) and a spontaneously immortalized human epithelial keratinocyte (HaCaT) cell line. It is demonstrated, using the Alamar Blue assay, that both cell models exhibit enhanced viability in collagen matrices compared to quartz substrates, commonly used for Raman spectroscopy. Using principal component analysis, it is shown that the Raman spectral analysis of cells in collagen matrices is minimally contaminated by substrate contributions and the cell to cell spectral variations are greatly reduced compared to those measured on quartz substrates. Furthermore, the spectral measurements are seen to have little contribution from the cell culture medium, implying that cultures can be kept viable over prolonged measurement or mapping procedures.

Graphical abstract: Three dimensional collagen gels as a cell culture matrix for the study of live cells by Raman spectroscopy

Article information

Article type
Paper
Submitted
03 Feb 2010
Accepted
09 Apr 2010
First published
30 Apr 2010

Analyst, 2010,135, 1697-1703

Three dimensional collagen gels as a cell culture matrix for the study of live cells by Raman spectroscopy

F. Bonnier, A. D. Meade, S. Merzha, P. Knief, K. Bhattacharya, F. M. Lyng and H. J. Byrne, Analyst, 2010, 135, 1697 DOI: 10.1039/C0AN00060D

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements