Issue 7, 2011
From the journal:

Analyst

Cetirizine as pH-dependent cross-reactant in a carbamazepine-specific immunoassay†

Arnold Bahlmann,a   Jana Falkenhagen,a   Michael G. Weller,a   Ulrich Panneab  and  Rudolf J. Schneider*a  

* Corresponding authors

a BAM Federal Institute for Materials Research and Testing, Richard-Willstätter-Str. 11, Berlin, Germany
E-mail: rudolf.schneider@bam.de
Fax: +49 3081041157
Tel: +49 3081041151

b Humboldt-Universität zu Berlin, Department of Chemistry, Brook-Taylor-Str. 2, Berlin, Germany

Abstract

High performance liquid chromatography (HPLC) was hyphenated with a previously reported carbamazepine-specific enzyme-linked immunosorbent assay (ELISA) as a screening approach to water analysis in order to identify possible interferences from transformation products. Treated wastewater was analysed and three substances were recognized by the antibody besides carbamazepine: the metabolites 10,11-dihydro-10,11-epoxycarbamazepine and 2-hydroxycarbamazepine plus the structurally not obviously related antihistamine cetirizine. The molar cross-reactivity against cetirizine was found to be pH-dependent and assessed to be 400% at pH 4.5 and 22% at pH 10.5. Performing the ELISA at pH 10.5 greatly improved the accuracy when carbamazepine was determined in surface and wastewater samples.

Graphical abstract: Cetirizine as pH-dependent cross-reactant in a carbamazepine-specific immunoassay

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Article information

DOI
https://doi.org/10.1039/C0AN00928H
Article type
Paper
Submitted
19 Nov 2010
Accepted
26 Jan 2011
First published
17 Feb 2011

Analyst, 2011,136, 1357-1364

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Cetirizine as pH-dependent cross-reactant in a carbamazepine-specific immunoassay

A. Bahlmann, J. Falkenhagen, M. G. Weller, U. Panne and R. J. Schneider, Analyst, 2011, 136, 1357 DOI: 10.1039/C0AN00928H

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