Issue 30, 2010

A method to site-specifically introduce methyllysine into proteins in E. coli

Abstract

A mutant pyrrolysyl-tRNA synthetase/tRNA pair was used to genetically encode allylcarbamoyl methyllysine in bacteria. This amino acid can be converted to methyllysine with a ruthenium catalyst, providing a straightforward approach for site-specifically introducing methyllysine residues into proteins.

Graphical abstract: A method to site-specifically introduce methyllysine into proteins in E. coli

Supplementary files

Article information

Article type
Communication
Submitted
24 Feb 2010
Accepted
07 Jun 2010
First published
22 Jun 2010

Chem. Commun., 2010,46, 5506-5508

A method to site-specifically introduce methyllysine into proteins in E. coli

H. Ai, J. W. Lee and P. G. Schultz, Chem. Commun., 2010, 46, 5506 DOI: 10.1039/C0CC00108B

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