Issue 5, 2011
From the journal:

Chemical Communications

Bead diffusion assay for discovering antimicrobial cyclic peptides†

Viviana S. Fluxà,a   Noélie Maillard,a   Malcolm G. P. Pageb  and  Jean-Louis Reymond*a  

* Corresponding authors

a Department of Chemistry and Biochemistry, University of Berne, Freiestrasse 3, CH-3012 Berne, Switzerland
E-mail: jean-louis.reymond@ioc.unibe.ch
Fax: +41 31 631 80 57
Tel: +41 31 631 43 25

b Basilea Pharmaceutica International Ltd., Switzerland

Abstract

A combinatorial library of 15 536 cyclic decapeptide analogues of tyrocidine A and gramicidin S was prepared on photocleavable TentaGel beads. The beads were photolyzed without solvent, and spread onto an agar plate inoculated with bacterial lawn. Clear zones were formed around beads carrying antimicrobially active peptides such as E18 c(kVOrnLfThiYOrnLq), which inhibited growth of B. subtilis and selected MRSA strains.

Graphical abstract: Bead diffusion assay for discovering antimicrobial cyclic peptides

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Article information

DOI
https://doi.org/10.1039/C0CC04670A
Article type
Communication
Submitted
28 Oct 2010
Accepted
22 Nov 2010
First published
10 Dec 2010

Chem. Commun., 2011,47, 1434-1436

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Bead diffusion assay for discovering antimicrobial cyclic peptides

V. S. Fluxà, N. Maillard, M. G. P. Page and J. Reymond, Chem. Commun., 2011, 47, 1434 DOI: 10.1039/C0CC04670A

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