Issue 23, 2010
From the journal:

Lab on a Chip

Centrifugal microfluidic system for primary amplification and secondary real-time PCR†

Maximilian Focke,a   Fabian Stumpf,a   Günter Roth,ab   Roland Zengerle*abc  and  Felix von Stettenab  

* Corresponding authors

a Laboratory for MEMS Applications, Department of Microsystems Engineering (IMTEK), University of Freiburg, Georges-Koehler-Allee 106, Freiburg, Germany
E-mail: zengerle@imtek.de

b HSG-IMIT, Wilhelm-Schickard-Straße 10, Villingen-Schwenningen, Germany

c Centre for biological signalling studies (bioss), University of Freiburg, Germany

Abstract

Pre-amplification is a basis for numerous polymerase chain reaction (PCR) protocols but bears severe contamination risks due to handling of high-copy DNA samples. Therefore we developed a self-contained centrifugal microfluidic system comprising pre-stored reagents; it enables pre-amplification of specific DNA sequences prior to automated aliquoting and real-time PCR in a modified commercial thermocycler.

Graphical abstract: Centrifugal microfluidic system for primary amplification and secondary real-time PCR

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Article information

DOI
https://doi.org/10.1039/C0LC00161A
Article type
Communication
Submitted
01 Jul 2010
Accepted
14 Sep 2010
First published
11 Oct 2010

Lab Chip, 2010,10, 3210-3212

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Centrifugal microfluidic system for primary amplification and secondary real-time PCR

M. Focke, F. Stumpf, G. Roth, R. Zengerle and F. von Stetten, Lab Chip, 2010, 10, 3210 DOI: 10.1039/C0LC00161A

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