Investigating the structural biofunctionality of antibodies conjugated to magnetic nanoparticles

Emanuela Occhipinti; Paolo Verderio; Antonino Natalello; Elisabetta Galbiati; Miriam Colombo; Serena Mazzucchelli; Agnese Salvadè; Paolo Tortora; Silvia Maria Doglia; Davide Prosperi

doi:10.1039/C0NR00436G

Investigating the structural biofunctionality of antibodies conjugated to magnetic nanoparticles†

Emanuela Occhipinti,‡^a Paolo Verderio,‡^b Antonino Natalello,^a Elisabetta Galbiati,^a Miriam Colombo,^ab Serena Mazzucchelli,^b Agnese Salvadè,^b Paolo Tortora,^a Silvia Maria Doglia^a and Davide Prosperi*^ab

Author affiliations

* Corresponding authors

^a Dipartimento di Biotecnologie e Bioscienze, Università di Milano-Bicocca, P.za della Scienza 2, Milano, Italy
E-mail: davide.prosperi@unimib.it
Fax: +39 026448 3565
Tel: +39 026448 3302

^b Centro di Microscopia Elettronica per le Nanotecnologie applicate alla medicina, Università di Milano, Via G.B. Grassi 74, Milano, Italy

Abstract

We present the synthesis of trastuzumab-functionalized pegylated iron oxide nanoparticles and provide an FTIR-based approach to gain a direct evidence of the actual conservation of the native structure of conjugated antibody. Their target-selectivity to specific cancer cell receptors has been also assessed.

This article is part of the themed collection: Surface nanotechnology for biological applications

Nanoscale

Investigating the structural biofunctionality of antibodies conjugated to magnetic nanoparticles†

Abstract

Supplementary files

Article information

Download Citation

Permissions

Investigating the structural biofunctionality of antibodies conjugated to magnetic nanoparticles

Social activity

Search articles by author

Spotlight

Advertisements