Issue 4, 2011

DNA-programmed spatial screening of carbohydrate–lectin interactions

Abstract

A wide range of multivalent scaffolds was assembled by using only five different PNA oligomers and various DNA templates. The flexibility of the PNA–DNA duplexes could be increased by introducing nick-sites and partially unpaired regions, as confirmed by MD simulations. The self-organized glyco-assemblies were used in a spatial screening of accessible carbohydrate binding sites in the Erythrina cristagalli lectin (ECL). This systematic investigation revealed a distance dependence which is in agreement with the crystal structure analysis.

Graphical abstract: DNA-programmed spatial screening of carbohydrate–lectin interactions

Supplementary files

Article information

Article type
Edge Article
Submitted
10 Nov 2010
Accepted
22 Dec 2010
First published
25 Jan 2011

Chem. Sci., 2011,2, 770-775

DNA-programmed spatial screening of carbohydrate–lectin interactions

C. Scheibe, A. Bujotzek, J. Dernedde, M. Weber and O. Seitz, Chem. Sci., 2011, 2, 770 DOI: 10.1039/C0SC00565G

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