Issue 1, 2011

Directed co-assembly of hemeproteins with amphiphilic block copolymers toward functional biomolecular materials

Abstract

Directed co-assembly of block copolymers and proteins/peptides may lead to hierarchically structured functional biomolecular materials. However, this requires one to synergistically direct multiple self-assembly processes. Retaining proper cofactor binding is essential to utilize many bio-motifs for catalytic reactions and sensing. Here, by using a heme-binding helix bundle peptide-polymer conjugate and a holomyoglobin-polymer conjugate as examples, we show that the simultaneous, macroscopic assembly of heme-binding proteins and diblock copolymers can be achieved in thin films without compromising protein structures, cofactor binding and enzymatic activities. To our knowledge, this is the first example of a protein/cofactor complex formed upon being co-assembled with an amphiphilic block copolymer in thin films. Molecular assemblyvia a combination of biomolecular recognition and polymer phase separation in this fashion will lead to hybrid materials combining properties of both synthetic and biological building blocks.

Graphical abstract: Directed co-assembly of heme proteins with amphiphilic block copolymers toward functional biomolecular materials

Supplementary files

Article information

Article type
Paper
Submitted
14 Aug 2010
Accepted
27 Sep 2010
First published
21 Oct 2010

Soft Matter, 2011,7, 172-179

Directed co-assembly of heme proteins with amphiphilic block copolymers toward functional biomolecular materials

A. D. Presley, J. J. Chang and T. Xu, Soft Matter, 2011, 7, 172 DOI: 10.1039/C0SM00817F

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements