Issue 22, 2011
From the journal:

Chemical Communications

Fast copper-free click DNA ligation by the ring-strain promoted alkyne-azide cycloaddition reaction†

Montserrat Shelbourne,a   Xiong Chen,a   Tom Brown‡*a  and  Afaf H. El-Sagheer‡*ab  

* Corresponding authors

a School of Chemistry, University of Southampton, Highfield, Southampton, UK
E-mail: ahes@soton.ac.uk, tb2@soton.ac.uk

b Chemistry Branch, Dept. of Science and Mathematics, Faculty of Petroleum and Mining Engineering, Suez Canal University, Suez 43721, Egypt

Abstract

Templated DNA strand ligation by the ring-strain promoted alkyne–azide [3+2] cycloaddition reaction is very fast; with dibenzocyclooctyne, the reaction is essentially complete in 1 min. It is inhibited by the presence of a single mismatched base pair suggesting applications in genetic analysis.

Graphical abstract: Fast copper-free click DNA ligation by the ring-strain promoted alkyne-azide cycloaddition reaction

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Article information

DOI
https://doi.org/10.1039/C1CC10743G
Article type
Communication
Submitted
08 Feb 2011
Accepted
12 Apr 2011
First published
06 May 2011

Chem. Commun., 2011,47, 6257-6259

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Fast copper-free click DNA ligation by the ring-strain promoted alkyne-azide cycloaddition reaction

M. Shelbourne, X. Chen, T. Brown and A. H. El-Sagheer, Chem. Commun., 2011, 47, 6257 DOI: 10.1039/C1CC10743G

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