Issue 26, 2011

Engineering of a bis-chelator motif into a protein α-helix for rigid lanthanide binding and paramagnetic NMR spectroscopy

Abstract

Attachment of two nitrilotriacetic acid-based ligands to a protein α-helix in an i, i + 4 configuration produces an octadentate chelating motif that is able to bind paramagnetic lanthanide ions rigidly and with high affinity, leading to large pseudocontact shifts and residual dipolar couplings in the NMR spectrum.

Graphical abstract: Engineering of a bis-chelator motif into a protein α-helix for rigid lanthanide binding and paramagnetic NMR spectroscopy

Supplementary files

Article information

Article type
Communication
Submitted
03 Apr 2011
Accepted
12 May 2011
First published
27 May 2011

Chem. Commun., 2011,47, 7368-7370

Engineering of a bis-chelator motif into a protein α-helix for rigid lanthanide binding and paramagnetic NMR spectroscopy

J. D. Swarbrick, P. Ung, X. Su, A. Maleckis, S. Chhabra, T. Huber, G. Otting and B. Graham, Chem. Commun., 2011, 47, 7368 DOI: 10.1039/C1CC11893E

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