Issue 28, 2011
From the journal:

Chemical Communications

Facile fabrication of recyclable and active nanobiocatalyst: purification and immobilization of enzyme in one pot with Ni-NTA functionalized magnetic nanoparticle†

Wen Wang,ab   Daniel I. C. Wangbc  and  Zhi Li*ab  

* Corresponding authors

a Department of Chemical and Biomolecular Engineering, National University of Singapore, 4 Engineering Drive 4, Singapore 117576
E-mail: chelz@nus.edu.sg
Fax: 65-6779 1936
Tel: 65-6516 8416

b Singapore-MIT Alliance, 4 Engineering Drive 3, Singapore 117576

c Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, USA

Abstract

Ni-NTA functionalized iron oxide magnetic nanoparticle was synthesized and used to selectively immobilize a his-tagged enzyme from cell free extract as an active and recyclable nanobiocatalyst, where purification and immobilization of the target enzyme were accomplished in one pot.

Graphical abstract: Facile fabrication of recyclable and active nanobiocatalyst: purification and immobilization of enzyme in one pot with Ni-NTA functionalized magnetic nanoparticle

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Article information

DOI
https://doi.org/10.1039/C1CC12685G
Article type
Communication
Submitted
06 May 2011
Accepted
02 Jun 2011
First published
20 Jun 2011

Chem. Commun., 2011,47, 8115-8117

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Facile fabrication of recyclable and active nanobiocatalyst: purification and immobilization of enzyme in one pot with Ni-NTA functionalized magnetic nanoparticle

W. Wang, D. I. C. Wang and Z. Li, Chem. Commun., 2011, 47, 8115 DOI: 10.1039/C1CC12685G

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