Issue 23, 2011

Co-encapsulation of enzyme and sensitive dye as a tool for fabrication of microcapsule based sensor for urea measuring

Abstract

Enzyme based micron sized sensing system with optical readout was fabricated by co-encapsulation of urease and dextran couple with pH sensitive dye SNARF-1 into polyelectrolyte multilayer capsules. Co-precipitation of calcium carbonate, urease and dextran followed up by multilayer film coating and Ca-extracting by EDTA resulted in the formation of 3.5–4 micron capsules, what enable the calibrated fluorescence response to urea in concentration range from 10−6 to 10−1 M. The presence of urea can be monitored on a single capsule level as illustrated by confocal fluorescent microscopy. Variations in urease:dye ratio in capsules, applicability and limits of use of that type multi-component microencapsulated sensors are discussed.

Graphical abstract: Co-encapsulation of enzyme and sensitive dye as a tool for fabrication of microcapsule based sensor for urea measuring

Article information

Article type
Paper
Submitted
09 Feb 2011
Accepted
12 Apr 2011
First published
13 May 2011

Phys. Chem. Chem. Phys., 2011,13, 11110-11117

Co-encapsulation of enzyme and sensitive dye as a tool for fabrication of microcapsule based sensor for urea measuring

L. I. Kazakova, L. I. Shabarchina and G. B. Sukhorukov, Phys. Chem. Chem. Phys., 2011, 13, 11110 DOI: 10.1039/C1CP20354A

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