Supramolecular assembly of hoechst-33258 with cucurbit[7]uril macrocycle

Abstract

Molecular assemblies of potential guest molecules through non-covalent host–guest interactions have found immense use in many applied areas. In this study supramolecular interaction of a biologically important dye Hoechst-33258 (H33258) has been investigated in aqueous solutions at different pHs, in the presence of a macrocyclic host, namely, cucurbit[7]uril (CB7). The pH dependent emission behaviour of H33258 is inherently connected with its protolytic equilibria which allow the dye in different geometrical conformations. This pH dependent structural orientation is greatly affected by the complexation with CB7. The significant structural changes in the monocationic H33258 brought out by CB7 at pH 7 have been documented in the fluorescence emission and lifetime data, which are comparatively less affected in case of the dicationic form, which is prominent in dye solutions at pH 4.5. The strong ion-dipole interactions provided by the carbonyl portals of the CB7 host adequately stabilize the CB7-H33258 complex, both in 1 : 1 and 2 : 1 stoichiometries at both the pH conditions. The Job's plot method, fluorescence anisotropy, NMR measurements and geometry optimization calculations confirm the stoichiometric arrangement and are found to be tunable with the addition of metal ions. The non-covalently stabilized assembly brings out large enhancement in the fluorescence emission due to the unique structural orientation attained by H33258, which reduces the non-radiative relaxation pathways. Comparison of the spectral data of the dye at different pH conditions in the absence and presence of CB7 proposes a large upward pK_a shift due to CB7 encapsulation, thus providing a handy tool to modulate the photophysical characteristics of the guest molecules.