Issue 14, 2011

High throughput label-free platform for statistical bio-molecular sensing

Abstract

Sensors are crucial in many daily operations including security, environmental control, human diagnostics and patient monitoring. Screening and online monitoring require reliable and high-throughput sensing. We report on the demonstration of a high-throughput label-free sensor platform utilizing cantilever based sensors. These sensors have often been acclaimed to facilitate highly parallelized operation. Unfortunately, so far no concept has been presented which offers large datasets as well as easy liquid sample handling. We use optics and mechanics from a DVD player to handle liquid samples and to read-out cantilever deflection and resonant frequency. Also, surface roughness is measured. When combined with cantilever deflection the roughness is discovered to hold valuable additional information on specific and unspecific binding events. In a few minutes, 30 liquid samples can be analyzed in parallel, each by 24 cantilever-based sensors. The approach was used to detect the binding of streptavidin and antibodies.

Graphical abstract: High throughput label-free platform for statistical bio-molecular sensing

Supplementary files

Article information

Article type
Paper
Submitted
09 Feb 2011
Accepted
26 Apr 2011
First published
27 May 2011

Lab Chip, 2011,11, 2411-2416

High throughput label-free platform for statistical bio-molecular sensing

F. G. Bosco, E. Hwu, C. Chen, S. Keller, M. Bache, M. H. Jakobsen, I. Hwang and A. Boisen, Lab Chip, 2011, 11, 2411 DOI: 10.1039/C1LC20116F

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