Issue 19, 2011

Rapid mixing of sub-microlitre drops by magnetic micro-stirring

Abstract

We demonstrate rapid mixing of sub-microlitre droplets (250 nl) using miniaturized magnetic stir bars (400 μm × 200 μm × 15 μm). The stir bars are fabricated using laser micromachining and placed on the substrate on which the drops are manipulated. They are activated by an externally applied magnetic field and used in combination with on-demand drop merging in enthalpy arrays. This technique results in a 10-fold increase in mixing rate, and a mixing time constant of about 2 s. Drop mixing times are measured by Förster resonance energy transfer (FRET) and verified by thermodynamic measurements of binding and enzymatic reactions.

Graphical abstract: Rapid mixing of sub-microlitre drops by magnetic micro-stirring

Supplementary files

Article information

Article type
Paper
Submitted
26 Apr 2011
Accepted
18 Jul 2011
First published
15 Aug 2011

Lab Chip, 2011,11, 3313-3319

Rapid mixing of sub-microlitre drops by magnetic micro-stirring

D. De Bruyker, M. I. Recht, A. A. S. Bhagat, F. E. Torres, A. G. Bell and R. H. Bruce, Lab Chip, 2011, 11, 3313 DOI: 10.1039/C1LC20354A

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