Issue 19, 2011

A digital microfluidic method for dried blood spot analysis

Abstract

Blood samples stored as dried blood spots (DBSs) are emerging as a useful sampling and storage vehicle for a wide range of applications. Unfortunately, the surging popularity of DBS samples has not yet been accompanied by an improvement in automated techniques for extraction and analysis. As a first step towards overcoming this challenge, we have developed a prototype microfluidic system for quantification of amino acids in dried blood spots, in which analytes are extracted, mixed with internal standards, derivatized, and reconstituted for analysis by (off-line and in-line) tandem mass spectrometry. The new method is fast, robust, precise, and most importantly, compatible with automation. We propose that the new method can potentially contribute to a new generation of analytical techniques for quantifying analytes in DBS samples for a wide range of applications.

Graphical abstract: A digital microfluidic method for dried blood spot analysis

Supplementary files

Article information

Article type
Paper
Submitted
15 Jun 2011
Accepted
03 Aug 2011
First published
25 Aug 2011

Lab Chip, 2011,11, 3218-3224

A digital microfluidic method for dried blood spot analysis

M. J. Jebrail, H. Yang, J. M. Mudrik, N. M. Lafrenière, C. McRoberts, O. Y. Al-Dirbashi, L. Fisher, P. Chakraborty and A. R. Wheeler, Lab Chip, 2011, 11, 3218 DOI: 10.1039/C1LC20524B

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