Issue 1, 2012
From the journal:

Lab on a Chip

Isolation of prostate tumor initiating cells (TICs) through their dielectrophoretic signature

Alireza Salmanzadeh,ab   Lina Romero,c   Hadi Shafiee,d   Roberto C. Gallo-Villanueva,a   Mark A. Stremler,b   Scott D. Cramerc  and  Rafael V. Davalos*ab  

* Corresponding authors

a Bioelectromechanical Systems Laboratory, School of Biomedical Engineering and Sciences, Virginia Tech–Wake Forest University, Blacksburg, VA, USA
E-mail: davalos@vt.edu
Tel: +1-540-998-9197

b Engineering Science and Mechanics Department, Virginia Tech, Blacksburg, VA, USA

c Department of Pharmacology, University of Colorado Denver, Aurora, CO, USA

d Center for Biomedical Engineering, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Boston, MA, USA

Abstract

In this study, the dielectrophoretic response of prostate tumor initiating cells (TICs) was investigated in a microfluidic system utilizing contactless dielectrophoresis (cDEP). The dielectrophoretic response of prostate TICs was observed to be distinctively different than that for non-TICs, enabling them to be sorted using cDEP. Culturing the sorted TICs generated spheroids, indicating that they were indeed initiating cells. This study presents the first marker-free TIC separation from non-TICs utilizing their electrical fingerprints through dielectrophoresis.

Graphical abstract: Isolation of prostate tumor initiating cells (TICs) through their dielectrophoretic signature

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Article information

DOI
https://doi.org/10.1039/C1LC20701F
Article type
Paper
Submitted
29 Jul 2011
Accepted
20 Oct 2011
First published
09 Nov 2011

Lab Chip, 2012,12, 182-189

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Isolation of prostate tumor initiating cells (TICs) through their dielectrophoretic signature

A. Salmanzadeh, L. Romero, H. Shafiee, R. C. Gallo-Villanueva, M. A. Stremler, S. D. Cramer and R. V. Davalos, Lab Chip, 2012, 12, 182 DOI: 10.1039/C1LC20701F

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