Issue 9, 2011
From the journal:

Nanoscale

Growth of immobilized DNA by polymerase: bridging nanoelectrodes with individual dsDNA molecules

Veikko Linko,*a   Jenni Leppiniemi,b   Boxuan Shen,a   Einari Niskanen,†c   Vesa P. Hytönenb  and  J. Jussi Topparia  

* Corresponding authors

a Nanoscience Center, Department of Physics, University of Jyväskylä, P.O. Box 35, Jyväskylä, Finland
E-mail: veikko.linko@jyu.fi
Fax: +358 14 260 4756
Tel: +358 14 260 4722

b Institute of Biomedical Technology, University of Tampere and Tampere University Hospital, Tampere, Finland

c Nanoscience Center, Department of Biological and Environmental Science, University of Jyväskylä, P.O. Box 35, Jyväskylä, Finland

Abstract

We present a method for controlled connection of gold electrodes with dsDNA molecules (locally on a chip) by utilizing polymerase to elongate single-stranded DNA primers attached to the electrodes. Thiol-modified oligonucleotides are directed and immobilized to nanoscale electrodes by means of dielectrophoretic trapping, and extended in a procedure mimicking PCR, finally forming a complete dsDNA molecule bridging the gap between the electrodes. The technique opens up opportunities for building from the bottom-up, for detection and sensing applications, and also for molecular electronics.

Graphical abstract: Growth of immobilized DNA by polymerase: bridging nanoelectrodes with individual dsDNA molecules

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Article information

DOI
https://doi.org/10.1039/C1NR10518C
Article type
Paper
Submitted
20 May 2011
Accepted
23 Jun 2011
First published
03 Aug 2011

Nanoscale, 2011,3, 3788-3792

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Growth of immobilized DNA by polymerase: bridging nanoelectrodes with individual dsDNA molecules

V. Linko, J. Leppiniemi, B. Shen, E. Niskanen, V. P. Hytönen and J. J. Toppari, Nanoscale, 2011, 3, 3788 DOI: 10.1039/C1NR10518C

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