Issue 1, 2012

Impedimetric thrombin aptasensor based on chemically modified graphenes

Abstract

Highly sensitive biosensors are of high importance to the biomedical field. Graphene represents a promising transducing platform for construction of biosensors. Here for the first time we compare the biosensing performance of a wide set of graphenes prepared by different methods. In this work, we present a simple and label-free electrochemical impedimetric aptasensor for thrombin based on chemically modified graphene (CMG) platforms such as graphite oxide (GPO), graphene oxide (GO), thermally reduced graphene oxide (TR-GO) and electrochemically reduced graphene oxide (ER-GO). Disposable screen-printed electrodes were first modified with chemically modified graphene (CMG) materials and used to immobilize a DNA aptamer which is specific to thrombin. The basis of detection relies on the changes in impedance spectra of redox probe after the binding of thrombin to the aptamer. It was discovered that graphene oxide (GO) is the most suitable material to be used as compared to the other three CMG materials. Furthermore, the optimum concentration of aptamer to be immobilized onto the modified electrode surface was determined to be 10 μM and the linear detection range of thrombin was 10–50 nM. Lastly, the aptasensor was found to demonstrate selectivity for thrombin. Such simply fabricated graphene oxide aptasensor shows high promise for clinical diagnosis of biomarkers and point-of-care analysis.

Graphical abstract: Impedimetric thrombin aptasensor based on chemically modified graphenes

Article information

Article type
Paper
Submitted
28 Jul 2011
Accepted
27 Sep 2011
First published
08 Nov 2011

Nanoscale, 2012,4, 143-147

Impedimetric thrombin aptasensor based on chemically modified graphenes

A. H. Loo, A. Bonanni and M. Pumera, Nanoscale, 2012, 4, 143 DOI: 10.1039/C1NR10966A

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