Issue 10, 2011
From the journal:

Photochemical & Photobiological Sciences

Chemical studies on the BRET system between the bioluminescence of Cypridina and quantum dots

Chun Wu,*a   Kosei Kawasaki,b   Satoru Ohgiyab  and  Yoshihiro Ohmiyac  

* Corresponding authors

a 1-8-31, Midorigaoka, Ikeda, Health Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Japan
E-mail: chun-wu@aist.go.jp
Fax: +81-72-751-9628
Tel: +81-72-751-7997

b 2-17-2-1Tsukisamu-Higashi, Toyohira-ku, Sapporo, Bio-production Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Japan

c Tsukuba Central 6, 1-1-1 Higashi, Tsukuba, Bio-production Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Japan

Abstract

We report a bioluminescence resonance energy transfer (BRET) system between Cypridina luciferase (Cluc) and QDs. After coupling of the biotinylated Cluc with streptavidin-QDs, a BRET emission of QDs through the resonance energy transfer from the oxidation of Vargula (Cypridina) luciferin was observed. A luciferin analogue having a naphtyl group at C6-position showed a large blue shift of the emission spectrum, which improved the ratio of this BRET system. After directly conjugating the luciferase with QDs, an efficient BRET was observed similar to the previous BRET using organic dyes. Furthermore, the symmetric emission spectra of QDs show a potential to realize multiplexed imaging based on the BRET of Cluc using the same luciferin.

Graphical abstract: Chemical studies on the BRET system between the bioluminescence of Cypridina and quantum dots

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Article information

DOI
https://doi.org/10.1039/C1PP05092C
Article type
Paper
Submitted
09 Mar 2011
Accepted
16 May 2011
First published
28 Jun 2011

Photochem. Photobiol. Sci., 2011,10, 1531-1534

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Chemical studies on the BRET system between the bioluminescence of Cypridina and quantum dots

C. Wu, K. Kawasaki, S. Ohgiya and Y. Ohmiya, Photochem. Photobiol. Sci., 2011, 10, 1531 DOI: 10.1039/C1PP05092C

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