Issue 2, 2012
From the journal:

Photochemical & Photobiological Sciences

Spectroscopic characterization of Venus at the single molecule level†

Charlotte C. David,a   Peter Dedecker,ab   Gert De Cremer,c   Natalie Verstraeten,c   Cyrielle Kint,c   Jan Michielsc  and  Johan Hofkens*a  

* Corresponding authors

a Department of Chemistry, Katholieke Universiteit Leuven, Celestijnenlaan 200F, Heverlee, Belgium
E-mail: johan.hofkens@chem.kuleuven.be
Fax: +32 1632 7418
Tel: +32 1632 7804

b Department of Pharmacology and Molecular Sciences, Johns Hopkins University School of Medicine, 725N. Wolfe Street, Baltimore, USA

c Department of Microbial and Molecular Systems, Katholieke Universiteit Leuven, Kasteelpark Arenberg 20, Heverlee, Belgium

Abstract

Venus is a recently developed, fast maturating, yellow fluorescent protein that has been used as a probe for in vivo applications. In the present work the photophysical characteristics of Venus were analyzed spectroscopically at the bulk and single molecule level. Through time-resolved single molecule measurements we found that single molecules of Venus display pronounced fluctuations in fluorescence emission, with clear fluorescence on- and off-times. These fluorescence intermittencies were found to occupy a broad range of time scales, ranging from milliseconds to several seconds. Such long off-times can complicate the analysis of single molecule counting experiments or single-molecule FRET experiments.

Graphical abstract: Spectroscopic characterization of Venus at the single molecule level

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Article information

DOI
https://doi.org/10.1039/C1PP05292F
Article type
Paper
Submitted
12 Sep 2011
Accepted
04 Nov 2011
First published
03 Jan 2012

Photochem. Photobiol. Sci., 2012,11, 358-363

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Spectroscopic characterization of Venus at the single molecule level

C. C. David, P. Dedecker, G. D. Cremer, N. Verstraeten, C. Kint, J. Michiels and J. Hofkens, Photochem. Photobiol. Sci., 2012, 11, 358 DOI: 10.1039/C1PP05292F

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